Coelomic Fluid of Earthworm, Eudrilus Eugeniae, Inhibits the Growth of Fungal Hyphae, in Vitro
Chandran Rajesh1, Kamaraj Rajamanikkam2, Ganapathy Nadana Raja Vadivu3, Karuppaiah Palanichelvam4
1Chandran Rajesh, Department of Biotechnology Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
2Kamaraj Rajamanikkam, Department of Biotechnology Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
3Ganapathy Nadana Raja Vadivu, Department of Biotechnology Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
4Karuppaiah Palanichelvam, Department of Biotechnology Engineering, Kalasalingam Academy of Research and Education College, Krishnankoil (Tamil Nadu), India.
Manuscript received on 24 November 2019 | Revised Manuscript received on 18 December 2019 | Manuscript Published on 30 December 2019 | PP: 792-796 | Volume-9 Issue-1S4 December 2019 | Retrieval Number: A11461291S419/19©BEIESP | DOI: 10.35940/ijeat.A1146.1291S419
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Knowledge on interactions between earthworms and microbes at molecular level has been obscure. Mechanism by which earthworms defend themselves against different microorganisms in the soil is largely unknown. Coelomic fluid from earthworm species has been shown to have antimicrobial activity. In this study we report that coelomic fluid isolated from Eudrilus eugeniae has antifungal activity against four different phytopathogens such as Rhizoctonia solani, Fusarium oxysporum, Aspergillus flavus and Verticillium dahlia. Incubation of coelomic fluid with fungal hyphae of all these four strains for 12 hours revealed that the growth of hyphae was greatly inhibited. Light microscopy studies confirmed that the hyphae growth was greatly reduced by incubation of coelomic fluid. Analysis of the fluid in the microscope showed the presence of different types of coelomocytes in the mucous fluid. Other molecules and compounds in the coelomic fluid might also have played role in antifungal activity.
Keywords: Earthworm, Eudrilus Eugeniae, Coelomocytes, Plant Fungal Pathogens, Antifungal.
Scope of the Article: Fluid Mechanics