Effect of Amplitude on Ultrasonic Assisted Extraction of Caffeic Acid from Andrographis Paniculata
Vi Vien Chia1, Sook Fun Pang2, JoliusGimbun3, Sureena Abdullah4, Mashitah M. Yusoff5
1ViVien Chia, Centre of Excellence for Advanced Research, Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Gambang, Pahang Malaysia.
2Sook Fun Pang, Centre of Excellence for Advanced Research, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Gambang, Pahang Malaysia.
3JoliusGimbun, Centre of Excellence for Advanced Research, Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang, Gambang, Pahang Malaysia.
4Sureena Abdullah, Centre of Excellence for Advanced Research, Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang Gambang, Pahang, Malaysia.
5Mashitah M. Yusoff, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Gambang, Pahang Malaysia.
Manuscript received on 29 May 2019 | Revised Manuscript received on 11 June 2019 | Manuscript Published on 22 June 2019 | PP: 524-527 | Volume-8 Issue-3S, February 2019 | Retrieval Number: C11120283S19/19©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Andrographispaniculata belongs to Acanthaceae family is often used as a traditional herbal medicine for treatment of diabetes and hypertension owing to the presence of medically useful bioactive compounds. The bioactive compounds from plant material must be extracted before it can be formulated as functional food. This paper presents the effect of sonication amplitude on the yield of caffeic acid extraction from A. paniculata. At first, the effect of particle size, solvent type and solid to solvent ratio was studied using a one-factor-at-time method in an ultrasonic assisted extraction. The extracts were quantified and identified using ultra-performance liquid chromatography coupled with photodiode array detector. The highest yield of caffeic acid (5.53 mg/g DW) was found using the particle size of 125-250 µm, solvent made of 50% aqueous ethanol and the solvent concentration, solid solvent ratio of 0.05g/mL and at sonication amplitude of 50%. Findings from this work may provide a useful guide to enhance extraction of caffeic acid from A. paniculata
Keywords: Andrographis Paniculata, Ultrasonic Extraction, Amplitude, Caffeic Acid.
Scope of the Article: Bio-Science and Bio-Technology