Differentiation of Oral Streptococcal Species by Haemolysis in Blood Agar Medium in Vitro
Ruchi Sharma1, Aditi Gupta2
1Ruchi Sharma, Mahatma Jyoti Rao Phoole University, Jaipur, India.
2Aditi Gupta, Mahatma Jyoti Rao Phoole University, Jaipur, India.
Manuscript received on March 24, 2014. | Revised Manuscript received on April 14, 2014. | Manuscript published on April 30, 2014. | PP: 143-144 | Volume-3, Issue-4, April 2014. | Retrieval Number: D2934043414/2013©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Pathogenic bacteria also cause infections such as tetanus, typhoid fever, diphtheria, syphilis, and leprosy. Blood agar medium contain a typical nutrient growth medium enriched with 5% sheep blood. It is useful for encouraging growth of fastidious organisms. The Three Types of Lytic Activities seen on the plate are- clear zone around bacterial growth -RBC hemolyzed completely (Beta-hemolysis, pathogenic); green zone around growth -RBC partially hemolyzed (Alpha-hemolysis); no change around growth -RBC is not hemolyzed (Gamma-hemolysis or no hemolysis). The discrimination of oral bacteria specifically Streptococcal species can be done by haemolysis tests using blood agar medium. Other than ultramicroscopic and morphological studies this experiment also conveys the species differentiation due to its lytic activity. Thus, blood agar medium can be a useful medium for pathogenic bacterial survey and investigation. The present review has proved the importance of blood agar utilization in diagnostics and disease analysis.
Keywords: Bacteria, Streptococcus, Blood, Agar, Haemolysis.