Central Composite Design as a Tools for Optimization of Antioxidant Activity on Cocoa Shell Extract
Arief Huzaimi Md Yusof1, Siti Salwa Abd Gani2, Uswatun Hasanah Zaidan3, Mohd Izuan Effendi Halmi4
1Arief Huzaimi Md Yusof, Cocoa Board, Cocoa Innovative and Technology Halal Products Research Institute, Universiti Putra Malaysia, Infoport, Serdang, Selangor, Malaysia.
2Siti Salwa Abd Gani, Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
3Uswatun Hasanah Zaidan, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
4Mohd Izuan Effendi Halmi, Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Manuscript received on 27 September 2019 | Revised Manuscript received on 09 November 2019 | Manuscript Published on 22 November 2019 | PP: 169-173 | Volume-8 Issue-6S3 September 2019 | Retrieval Number: F10280986S319/19©BEIESP | DOI: 10.35940/ijeat.F1028.0986S319
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: This study seeks to analyze and optimize the antioxidant activity thru beta carotene bleaching assay influenced by different extraction condition on extracts obtained from Malaysia Cocoa Shell. The condition was optimized by response surface methodology with five levels of the model. There are three variables involved in this study, namely: ethanol concentration (70-90 v/v %), temperature (45-65 ºC), and ultrasound irradiation time (30-60 min). The optimum condition obtains for temperature, duration of extraction and solvent concentration were 55 ºC, 45 minutes and 63.18% respectively at a constant frequency of 40 kHz. In this condition, the antioxidant activity experimental was 98.91±0.5% and predicted 98.44%. The ANOVA shows the coefficient of determination (R2 ) and the lack of fit test was 0.9846 and 0.6105 respectively. The desirability function from T-test data, modeled equation fits the data, reveals that the design can be used to predicting future observation within the design range.
Keywords: Antioxidant, Cocoa Shells and Response Surface Methodology.
Scope of the Article: Composite Materials