Creating Biotechnological Hybrids of Sugar Beet
Alexey Viktorovich Logvinov1, Vladimir Nikolaevich Mishchenko2, Viktor Alekseevich Logvinov3, Viktor Vasilyevich Moiseev4, Arkady Viktorovich Moiseev5, Olga Viktorovna6
1Alexey Viktorovich Logvinov, Pervomajskaya Breeding and Experimental Station Sugar beets», Gulkevichi, Krasnodar Region, Russia.
2Vladimir Nikolaevich Mishchenko, Pervomajskaya Breeding and Experimental Station Sugar beets», Gulkevichi, Krasnodar Region, Russia.
3Viktor Alekseevich Logvinov, Pervomajskaya Breeding and Experimental Station Sugar beets», Gulkevichi, Krasnodar Region, Russia.
4Viktor Vasilyevich Moiseev, Kuban State Agrarian University Behalf of the I.T. Trubilina, Krasnodar, Russia.
5Arkady Viktorovich Moiseev, Kuban State Agrarian University Behalf of the I.T. Trubilina, Krasnodar, Russia.
6Olga Viktorovna Koshchaeva, Kuban State Agrarian University Behalf of the I.T. Trubilina, Krasnodar, Russia.
Manuscript received on July 20, 2019. | Revised Manuscript received on August 10, 2019. | Manuscript published on August 30, 2019. | PP: 5167-5175 | Volume-8 Issue-6, August 2019. | Retrieval Number: F9103088619/2019©BEIESP | DOI: 10.35940/ijeat.F9103.088619
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Currently, implementation of the breeding programs, including the commonly recognized areas and classic breeding methods, cannot sufficiently ensure a quick and significant increase in the productivity of sugar beet hybrids, since its gene pool is almost exhausted. Based on the achievements in the field of genetics, new approaches to and opportunities in creating highly productive agrocoenoses of sugar beet have become popular. As a result of many years of work, results have been obtained about the nature of inheriting the resistance to glyphosate in individual heterozygous apo- and syncarpous forms in case of inbreeding and pair mating with the MC tester. The expression of target genes in the generations was monitored by the survival rate of sugar beet plants after the treatment with glyphosate. During the research, individuals with a high level of gene expression were selected. Upon self-pollination of initial heterozygous original forms, deviations from Mendelian segregation were observed in most cases. The criterion for assessing the stability of expression of glyphosate resistance genes in case of seed breeding was the compliance with the laws of Mendel among the analyzed descendants. In the initial stages of the research, the level of stability gene expression had been 10 – 15 % of the total number of analyzed plants. After four self-pollinations, the stability gene expression significantly increased, and genotypes with the resistance of 91 – 100 % were selected. The first apo- and syncarpous self-pollinating lines of sugar beet with high tolerance in the role of resistance donors have been created. The positive results of preliminary tests of the first glyphosate-tolerant hybrids need confirmation. Seeds and roots of resistant forms have been obtained for further research.
Keywords: Gene Expression, Glyphosate, Hybrid, Profitability, Self-Pollination, Sugar Beet, Sugar Content, Test, Tolerance, Yield Rate, Weeds